EDUCATIONAL PROGRAMS TO ADDRESS THE ECONOMIC ADJUSTMENTS FACING TOBACCO FARMERS AND RURAL COMMUNITIES

This paper discusses the context within which educational programs tailored to tobacco producers and related rural communities have developed. Discussion is expanded by examining current program approaches employed by various organizations. Many of these organizations have a manual stake in helping producers in tobacco communities develop their management capacity. A range of initiatives aimed at facilitating economic adjustment is compared, including the major issues addressed and expected outcomes. Many of the initiatives have made useful contributions; however, gaps and limitations remain. These are considered as future educational efforts and issues are discussed.educational programs, tobacco producers, Community/Rural/Urban Development,

In Vitro Assessment of Developmental Neurotoxicity: Use of Microelectrode Arrays to Measure Functional Changes in Neuronal Network Ontogeny1

Because the Developmental Neurotoxicity Testing Guidelines require large numbers of animals and is expensive, development of in vitro approaches to screen chemicals for potential developmental neurotoxicity is a high priority. Many proposed approaches for screening are biochemical or morphological, and do not assess function of neuronal networks. In this study, microelectrode arrays (MEAs) were used to determine if chemical-induced changes in function could be detected by assessing the development of spontaneous network activity. MEAs record individual action potential spikes as well as groups of spikes (bursts) in neuronal networks, and activity can be assessed repeatedly over days in vitro (DIV). Primary cultures of rat cortical neurons were prepared on MEAs and spontaneous activity was assessed on DIV 2, 6, 9, 13, and 20 to determine the in vitro developmental profile of spontaneous spiking and bursting in cortical networks. In addition, 5 μM of the protein kinase C inhibitor bisindolylmaleamide-1 (Bis-1) was added to MEAs (n = 9–18) on DIV 5 to determine if changes in spontaneous activity could be detected in response to inhibition of neurite outgrowth. A clear profile of in vitro activity development occurred in control MEAs, with the number of active channels increasing from 0/MEA on DIV 2 to 37 ± 5/MEA by DIV 13; the rate of increase was most rapid between DIV 6 and 9, and activity declined by DIV 20. A similar pattern was observed for the number of bursting channels, as well as the total number of bursts. Bis-1 decreased the number of active channels/MEA and the number of bursting channels/MEA. Burst characteristics, such as burst duration and the number of spikes in a burst, were unchanged by Bis-1. These results demonstrate that MEAs can be used to assess the development of functional neuronal networks in vitro, as well as chemical-induced dysfunction

Characterization of Early Cortical Neural Network Development in Multiwell Microelectrode Array Plates.

We examined neural network ontogeny using microelectrode array (MEA) recordings made in multiwell MEA (mwMEA) plates over the first 12 days in vitro (DIV). In primary cortical cultures, action potential spiking activity developed rapidly between DIV 5 and 12. Spiking was sporadic and unorganized at early DIV, and became progressively more organized with time, with bursting parameters, synchrony, and network bursting increasing between DIV 5 and 12. We selected 12 features to describe network activity; principal components analysis using these features demonstrated segregation of data by age at both the well and plate levels. Using random forest classifiers and support vector machines, we demonstrated that four features (coefficient of variation [CV] of within-burst interspike interval, CV of interburst interval, network spike rate, and burst rate) could predict the age of each well recording with >65% accuracy. When restricting the classification to a binary decision, accuracy improved to as high as 95%. Further, we present a novel resampling approach to determine the number of wells needed for comparing different treatments. Overall, these results demonstrate that network development on mwMEA plates is similar to development in single-well MEAs. The increased throughput of mwMEAs will facilitate screening drugs, chemicals, or disease states for effects on neurodevelopment.EC was supported by a Wellcome Trust PhD studentship and NIHR Cambridge Biomedical Research Centre studentship. DH was supported by student services contract #EP-13-D-000108 and by a travelling fellowship from the Company of Biologists.This is the final version of the article. It first appeared from SAGE Publications via https://doi.org/10.1177/108705711664052

Minor versus major mergers: the stellar mass growth of massive galaxies from z=3 using number density selection techniques

We present a study on the stellar mass growth of the progenitors of local massive galaxies with a variety of number density selections with n≤1×10−4 Mpc−3 (corresponding to M*=1011.24 M⊙ at z=0.3) in the redshift range 0.3<z<3.0. We select the progenitors of massive galaxies using a constant number density selection, and one which is adjusted to account for major mergers. We find that the progenitors of massive galaxies grow by a factor of 4 in total stellar mass over this redshift range. On average the stellar mass added via the processes of star formation, major and minor mergers account for 24±8, 17±15 and 34±14per cent, respectively, of the total galaxy stellar mass at z=0.3. Therefore 51±20per cent of the total stellar mass in massive galaxies at z=0.3 is created externally to their z=3 progenitors. We explore the implication of these results on the cold gas accretion rate and size evolution of the progenitors of most massive galaxies over the same redshift range. We find an average gas accretion rate of∼66±32 M⊙ yr−1 over the redshift range of 1.5<z<3.0. We find that the size evolution of a galaxy sample selected this way is on average lower than the findings of other investigation

A consistent measure of the merger histories of massive galaxies using close-pair statistics I:Major mergers at z &lt;3.5

We use a large sample of $\sim 350,000$ galaxies constructed by combining the UKIDSS UDS, VIDEO/CFHT-LS, UltraVISTA/COSMOS and GAMA survey regions to probe the major merging histories of massive galaxies ($>10^{10}\ \mathrm{M}_\odot$) at $0.005 < z < 3.5$. We use a method adapted from that presented in Lopez-Sanjuan et al. (2014) using the full photometric redshift probability distributions, to measure pair $\textit{fractions}$ of flux-limited, stellar mass selected galaxy samples using close-pair statistics. The pair fraction is found to weakly evolve as $\propto (1+z)^{0.8}$ with no dependence on stellar mass. We subsequently derive major merger $\textit{rates}$ for galaxies at $> 10^{10}\ \mathrm{M}_\odot$ and at a constant number density of $n > 10^{-4}$ Mpc$^{-3}$, and find rates a factor of 2-3 smaller than previous works, although this depends strongly on the assumed merger timescale and likelihood of a close-pair merging. Galaxies undergo approximately 0.5 major mergers at $z < 3.5$, accruing an additional 1-4 $\times 10^{10}\ \mathrm{M}_\odot$ in the process. Major merger accretion rate densities of $\sim 2 \times 10^{-4}$ $\mathrm{M}_\odot$ yr$^{-1}$ Mpc$^{-3}$ are found for number density selected samples, indicating that direct progenitors of local massive ($>10^{11}\mathrm{M}_\odot$) galaxies have experienced a steady supply of stellar mass via major mergers throughout their evolution. While pair fractions are found to agree with those predicted by the Henriques et al. (2014) semi-analytic model, the Illustris hydrodynamical simulation fails to quantitatively reproduce derived merger rates. Furthermore, we find major mergers become a comparable source of stellar mass growth compared to star-formation at $z < 1$, but is 10-100 times smaller than the SFR density at higher redshifts.Comment: 26 pages, 18 figures, accepted to MNRA

Transcriptional response of rat frontal cortex following acute In Vivo exposure to the pyrethroid insecticides permethrin and deltamethrin

<p>Abstract</p> <p>Background</p> <p>Pyrethroids are neurotoxic pesticides that interact with membrane bound ion channels in neurons and disrupt nerve function. The purpose of this study was to characterize and explore changes in gene expression that occur in the rat frontal cortex, an area of CNS affected by pyrethroids, following an acute low-dose exposure.</p> <p>Results</p> <p>Rats were acutely exposed to either deltamethrin (0.3 – 3 mg/kg) or permethrin (1 – 100 mg/kg) followed by collection of cortical tissue at 6 hours. The doses used range from those that cause minimal signs of intoxication at the behavioral level to doses well below apparent no effect levels in the whole animal. A statistical framework based on parallel linear (SAM) and isotonic regression (PIR) methods identified 95 and 53 probe sets as dose-responsive. The PIR analysis was most sensitive for detecting transcripts with changes in expression at the NOAEL dose. A sub-set of genes (<it>Camk1g</it>, <it>Ddc</it>, <it>Gpd3</it>, <it>c-fos </it>and <it>Egr1</it>) was then confirmed by qRT-PCR and examined in a time course study. Changes in mRNA levels were typically less than 3-fold in magnitude across all components of the study. The responses observed are consistent with pyrethroids producing increased neuronal excitation in the cortex following a low-dose <it>in vivo </it>exposure. In addition, Significance Analysis of Function and Expression (SAFE) identified significantly enriched gene categories common for both pyrethroids, including some relating to branching morphogenesis. Exposure of primary cortical cell cultures to both compounds resulted in an increase (~25%) in the number of neurite branch points, supporting the results of the SAFE analysis.</p> <p>Conclusion</p> <p>In the present study, pyrethroids induced changes in gene expression in the frontal cortex near the threshold for decreases in ambulatory motor activity <it>in vivo</it>. The penalized regression methods performed similarly in detecting dose-dependent changes in gene transcription. Finally, SAFE analysis of gene expression data identified branching morphogenesis as a biological process sensitive to pyrethroids and subsequent <it>in vitro </it>experiments confirmed this predicted effect. The novel findings regarding pyrethroid effects on branching morphogenesis indicate these compounds may act as developmental neurotoxicants that affect normal neuronal morphology.</p